Cytotoxic effect of γ-sitosterol from Kejibeling (Strobilanthes crispus) and its mechanism of action towards c-myc gene expression and apoptotic pathway
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چکیده
Metode: Penelitian in vitro ini menggunakan kultur sel kanker kolon manusia (Caco-2), sel kanker hepar manusia (HepG2), sel kanker payudara tergantung hormon (MCF7) dan kultur sel normal hepar manusia (Chang Liver). Efek sitotoksik diukur melalui uji MTT melalui penentuan kadar yang membunuh 50% sel (IC50). Mekanisme sitotoksik dipelajari dengan menentukan pengaruh penambahan γ-sitosterol pada ekspresi gen c-myc menggunakan metode reverse transciptase-polymerase chain reaction (RT-PCR). Pengaruh γ-sitosterol melalui jalur apoptosis dipelajari dengan menggunakan asai terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL).
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تاریخ انتشار 2015